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Biomaterials & Analytical Validation Technician

Pear Bio

Pear Bio

London, UK
Posted on Friday, May 26, 2023
About Pear Bio
At Pear Bio, we are personalising cancer treatment selection because every cancer is unique. To achieve this, we’ve developed a test that cultures patient tumour samples and matched immune cells using a hydrogel-based system, monitors cell behaviours during therapy exposure, and eliminates ineffective treatments for that patient. We are a VC-backed start-up based in London. To grow our company, we’re looking for a biomaterials and analytical validation technician to join our early-stage team. Will you be the one?
Job Description
The biomaterials and analytical validation technician will support the Diagnostics R&D team through validation and further development of 3D cell culture architectures designed to test the efficacy of various therapies ex vivo. You will have access to patient tumour samples and matched whole blood to create 3D cell cultures using Pear’s ex vivo tumour culture + computer vision platform. You will test various hydrogel formulations to support the culture of primary cells and validate their usefulness through phenotypic assays (including molecular and biophysical assays). The model will be used to monitor relevant behaviours in 3D like immune cell infiltration, migration and tumour-cell killing over time. This role will also encompass analytical workflow validation, required to show the model’s robustness for clinical and regulatory applications.
Parameters such as lot-to-lot variability, shelf-life, temperature stability and biophysical properties will be assessed to characterise biomaterials within Pear Bio. In addition, this role will support the validation of in-house microfluidic chips and microphysiological plates for use in the Pear test. This will include integration of the hydrogel culture with Pear Bio’s hardware, imaging workflow and computer-vision readouts.

Job Responsibilities

  • Process primary tumour tissue and whole blood
  • Test physiologically relevant 3D models that co-culture cancer, stromal, immune and other cell types
  • Characterise 3D hydrogel-based cell cultures assessing their physical and biological features, using microscopy and molecular biology techniques
  • Operate and validate microfluidics and microphysiological devices for the testing of therapeutics
  • Conduct QA/QC and analytical validation for regulatory approval (FDA)
  • Support the Diagnostics team in translating biomaterials work to the clinical team
  • Work collaboratively and design experiments closely with biomaterial scientists, hardware engineers, tumour engineers, compound screening scientists and immuno-oncology scientists

Must-Haves

  • MSc in an engineering or life sciences field or equivalent relevant experience in an industry setting
  • Experience in tissue engineering (preferably oncology)
  • Expert in aseptic cell culture techniques
  • Experience in bioprocess validation and QA/QC methodologies (e.g. shelf-life testing, reporting)
  • Proficient with microscopy techniques like confocal microscopyImmunised for Hepatitis B (all 3 doses received over a 6 month period) or willing to receive the first of the dose of the vaccine prior to the commencement of work with the intention of completing all 3 doses within 6 months
  • Ability to work both independently or as part of a larger team as required and an appreciation of the flexibility required when working in a dynamic, fast-paced environment
  • Right to work in the UK

Nice-to-Haves

  • Primary tumour cell isolation and culture
  • Experience in 3D cell culture and microscopy, preferably with primary cells or patient tissue
  • Experience with biological assays such as DNA quantification, Cell-Titer Glo or Real-time Glo, and ELISAs
  • Experience in biomaterial characterisation (e.g. SEM, rheology, etc.)
  • Experience in preparing documentation for regulatory agencies
  • Knowledge of GxP practices (especially GMP)

What’s in It for You

  • Competitive compensation in line with industry standards
  • Stock options in a growing start-up28 days of annual leave excluding bank holidays and Christmas closure
  • Yearly personal development budget, plus the chance to represent the company at international conferences
  • Open work environment where your opinions are valued
  • High career growth & personal development in a fast-paced, dynamic environment
  • The chance to have an impact in shaping the future of an early-stage start-up
  • Company perks / discounts via Perks at Work
Please note
We are unable to sponsor work visas at this time. Please confirm your ability to work in the UK without visa sponsorship before applying. The position is not eligible for remote work, so you’ll be expected to be on-site.
How to Apply